DNA extraction | Quantification | Amplification DNA library preparation | DNA library validation Sequencing | Genome assembly
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Next Gen Sequencing Services
DNA extraction is the technique used to remove DNA from cell membranes, proteins, and other cellular components in a biological sample.
Quantification of nucleic material is commonly performed to determine the average concentration of DNA or RNA in a sample prior to proceeding with downstream NGS procedures.
Amplification of nucleic material through the polymerase chain reaction (PCR) technique is aimed to enhance/multiply the nucleic material for downstream NGS applications.
Library preparation is a very vital and key step of NGS, which allows DNA to be covalently linked through ligation to the sequencing adapters, barcoding indexes and specific adapters that hybridize with complement flow cell adaptors, to initiate the bridge amplification for Illumina chemistry.
DNA library validation entails determining the fragment sizes and quality of DNA library prior to sequencing step.
Sequencing refers to the general laboratory technique for determining the exact base (A, T, C and G) in a biomolecule such as DNA, RNA and deduced protein.
Genome assembly entails the computational process of deciphering the sequence composition of the genetic material (DNA), within the cell of an organism using numerous short sequences called reads to formulate continuous contigs that complete the genome for analysis.
DNA Library Preparation
The University of the Free State – Next Generation Sequencing (UFS-NGS) Unit is a business research facility under the Faculty of Health Sciences. It provides genomics services to researchers, upon request. In addition, it offers practical experience to visiting and appointed scientific interns.