Raw sequence data in form of short contigs are assembled in an overlapping arrangement, to complete the actual order of the sequence of genes and/or genomes of an organism, using bioinformatics software. There are two ways to assemble the raw sequence data. De novo assembly entails constructing a genome from scratch without the aid of genomic reference data. Reference-based genome assembly is the alternative assembly technique that aligns short reads to a reference genome that is used as a template. Upon request, the UFS-NGS Unit performs genome assembly (De novo and/or reference-based mapping) of the generated raw fastq files.
